Application

Detect Cdk4 with Anti-Cdk4 Antibody, clone DCS-35 (Mouse Monoclonal Antibody), that has been shown to work in IF, IP, EA, WB & IHC.

Research CategoryEpigenetics & Nuclear Function

Immunofluorescence

Immunoprecipitation: excellent for coprecipitation of D-type cyclins bound to cdk4.

Western Blotting: 2-4 ug/mL

Kinase Assay

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Immunogen

Raised against purified recombinant cdk4 protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABE548

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Format: Purified

Specificity

Recognizes a 34 kDa protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks.

Storage and Stability

Maintain liquid material at 2-8°C for up to 24 months.